Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: Oxidized low-density lipoprotein (oxLDL) induces proliferation in human umbilical vein endothelial cells (HUVECs) by CCK-8 assay. OxLDL at concentrations of 10 μg/ml, 25 μg/ml, 50 μg/ml, 75 μg/ml, 100 μg/ml were applied to HUVECs for 3 h (A), 6 h (B), 12 h (C), 24 h (D), 48 h (E) with basal conditions, and then the optical density (OD) at 450 nm was measured. Each data point represents mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01 compared with control. #P<0.05, ##P<0.01 compared with normal.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: The effects of native LDL and oxLDL on HUVEC. Both native LDL and oxLDL at concentrations of 10 μg/ml, 25 μg/ml, 50 μg/ml, 75 μg/ml, 100 μg/ml for 24 h were applied to HUVECs. The impact of native LDL or oxLDL on the morphological changes in HUVECs using phase-contrast microscope (×100) (A, B, respectively) and on the proliferation using CCK-8 (C). Each data point represents mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01 compared with control.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: Microscopy, CCK-8 Assay, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: Berberine inhibits proliferation of HUVEC induced by oxLDL. Pretreated with berberine at concentrations of 5 μg/ml, 10 μg/ml, 25 μg/ml, 50 μg/ml, attenuated the oxLDL-induced proliferation of HUVECs in a dose-dependent manner using CCK-8 assay (A). Berberine at concentration of 25 μg/ml significantly inhibited 50 μg/ml oxLDL-induced proliferation of HUVECs using EdU proliferation assay using a confocal laser scanning microscope (×100) (B) and the ratio of EdU-positive cells to total cells was shown (C). Each data point represents mean ± standard deviation of three independent experiments. **P<0.01 compared with control. #P<0.05, ##P<0.01 compared with oxLDL 50 μg/ml. $P<0.05 compared with Control.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: CCK-8 Assay, Concentration Assay, Proliferation Assay, Laser-Scanning Microscopy, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: Berberine significantly decreased PCNA, NF-кB, LOX-1 and PI3K mRNA expression induced by oxLDL in HUVECs in a dose-dependent manner. Real-time PCR assay for PCNA (A), NF-кB (B), LOX-1 (C) and PI3K (D), respectively. Each data point represents mean ± standard deviation of three independent experiments. **P<0.01 compared with control. ##P<0.01 compared with oxLDL 50 μg/ml.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: OxLDL induced the proliferation through up-regulating PCNA, LOX-1 and activating NF-кB, phospho-Akt, phospho-ERK, phospho-p38 signal pathways in a dose-dependent manner. Western blot to PCNA, NF-кB, LOX-1 (A, E, F, G), and phospho-Akt (B, H), phospho-ERK (C, I), phospho-p38 (D, J) in HUVECs. Each data point represents mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01 compared with control.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: Western Blot, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: Berberine inhibited oxLDL-induced HUVECs proliferation by down-regulating the PCNA, LOX-1 and inactivating NF-кB, phospho-Akt, phospho-ERK, phospho-p38 signal pathways. Western blot to PCNA, NF-кB, LOX-1 (A, E, F, G), and phospho-Akt (B, H), phospho-ERK (C, I), phospho-p38 (D, J) in HUVECs. Each data point represents mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01 compared with control. #P<0.05, ##P<0.01 compared with oxLDL 50 μg/ml.

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: Western Blot, Standard Deviation, Control

Journal: American Journal of Translational Research

Article Title: Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells

doi:

Figure Lengend Snippet: Berberine inhibited oxLDL-induced HUVECs proliferation through PI3k/Akt, ERK and p38MAPK signal pathways. HUVECs were pretreated with or without LY294002 (25 μM), PD98059 (10 μM) and SB202190 (10 μM) for 1 h followed by berberine (25 μg/ml) incubation with oxLDL (50 μg/ml) induction for 24 h. A-F: Western blot analysis showed a clear increase of Akt, ERK and p38 phosphorylation induced by LY294002, PD98059 and SB202190. G: CCK-8 assay showd that LY294002, PD98059 and SB202190 abrogated the inhibition of berberine in oxLDL-induced HUVECs proliferation. Each data point represents mean ± standard deviation of three independent experiments. **P<0.01 compared with control. #P<0.05, ##P<0.01 compared with oxLDL 50 μg/ml. $P<0.05, $$P<0.01, compared with berberine (25 μg/ml).

Article Snippet: Culture of HUVEC HUVECs were purchased from Sciencell and grown in M199 with 10% (v/v) FBS, 10 ng/ml ECGF and 100 U/ml penicillin-streptomycin (complete medium) at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air.

Techniques: Incubation, Western Blot, Phospho-proteomics, CCK-8 Assay, Inhibition, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Twist1 Controls Lung Vascular Permeability and Endotoxin-Induced Pulmonary Edema by Altering Tie2 Expression

doi: 10.1371/journal.pone.0073407

Figure Lengend Snippet: A ) Immunofluorescence micrographs showing cell-cell junction structure by VE-cadherin staining in L-HMVE cells treated with Twist1 siRNA #1 or #2 (bar, 50 µm). DAPI staining shows the nuclei of cells. Arrows show the region where cell-cell junctions are disrupted. As a control, cells were treated with siRNA duplex with an irrelevant sequence. B ) Graph showing the quantitation of the total discontinuous area of at least 10 fields (*, p<0.01). C ) Graph showing endothelial permeability in L-HMVE cells treated with control siRNA, Twist1 siRNA #1 or #2 (*, p<0.05). Permeability (Pa) values were evaluated after 6 h and are expressed as percentage of control cells. D ) Immunoblots showing tyrosine phosphorylated Tie2 detected by phosphotyrosine antibody (4G10) and total Tie2 immunoprecipitated with Tie2 antibody from total cell lysates in Twist1 knockdown (siRNA #1 treated) L-HMVE cells. E ) Levels of active RhoA in Twist1 knockdown (siRNA #1 treated) L-HMVE cells. Quantitative results (ratio of active RhoA to total RhoA) were normalized to control siRNA treated cells (*, p<0.01). F ) Graph showing endothelial permeability in HUVE cells treated with Twist1 siRNA #1, Tie2 DNA, or in combination (*, p<0.05). As a control, cells were treated with siRNA duplex with an irrelevant sequence and/or control DNA (vector only). Immunoblots showing Twist1, Tie2, and GAPDH protein levels in HUVE cells treated with Twist1 siRNA #1, Tie2 DNA, or in combination. All error bars are s.e.m.

Article Snippet: L-HMVE cells and HUVE cells (Lonza, Walkersville, MD) were cultured as described before [ , ].

Techniques: Immunofluorescence, Staining, Sequencing, Quantitation Assay, Permeability, Western Blot, Immunoprecipitation, Plasmid Preparation

Journal: Tissue Engineering and Regenerative Medicine

Article Title: A Novel Hypothesis and Characterization to Isolate Microvascular Endothelial Cells Simultaneously with Adipose-Derived Stem Cells from the Human Adipose-Derived Stromal Vascular Fraction

doi: 10.1007/s13770-021-00332-5

Figure Lengend Snippet: Comparative analysis of properties according to the HMVEC-A isolation method. A Morphology and tube formation of HMVEC-A isolated by the vascular fraction (VF) isolation, magnetic-activated cell sorting (MACS) isolation method, and fluorescence-activated cell sorting (FACS) isolation methods were compared with Lonza HUVEC and HMVEC by phase-contrast microscopy; magnification, 40 × . B The VF isolation method produced a significantly higher yield than the FACS isolation method and MACS isolation method (*p < 0.05, **p < 0.01, n = 3). The doubling time was in the order of VF isolation method, MACS isolation method and FACS isolation method, and VF showed the fastest doubling time, but there was no statistically significant difference. The error bars indicate the standard errors of the mean of triplicate measurements. C FACS verification of EC-specific marker expression according to each isolation method. Positivity for the endothelial-specific cell surface antigens CD31 (PECAM1) and CD146 (MCAM) is indicated. Cells were analyzed at passage 2 (n = 3)

Article Snippet: These vascular endothelial cells were positive for the surface markers CD31 and CD146 and exhibited an angiogenic potential very similar to that of the Lonza HUVEC and HMVEC cell lines.

Techniques: Isolation, FACS, Fluorescence, Microscopy, Produced, Marker, Expressing

Journal: Tissue Engineering and Regenerative Medicine

Article Title: A Novel Hypothesis and Characterization to Isolate Microvascular Endothelial Cells Simultaneously with Adipose-Derived Stem Cells from the Human Adipose-Derived Stromal Vascular Fraction

doi: 10.1007/s13770-021-00332-5

Figure Lengend Snippet: Comparative analysis of properties according to the HMVEC-A isolation method. A Morphology and tube formation of HMVEC-A isolated by the vascular fraction (VF) isolation, magnetic-activated cell sorting (MACS) isolation method, and fluorescence-activated cell sorting (FACS) isolation methods were compared with Lonza HUVEC and HMVEC by phase-contrast microscopy; magnification, 40 × . B The VF isolation method produced a significantly higher yield than the FACS isolation method and MACS isolation method (*p < 0.05, **p < 0.01, n = 3). The doubling time was in the order of VF isolation method, MACS isolation method and FACS isolation method, and VF showed the fastest doubling time, but there was no statistically significant difference. The error bars indicate the standard errors of the mean of triplicate measurements. C FACS verification of EC-specific marker expression according to each isolation method. Positivity for the endothelial-specific cell surface antigens CD31 (PECAM1) and CD146 (MCAM) is indicated. Cells were analyzed at passage 2 (n = 3)

Article Snippet: A Morphology and tube formation of HMVEC-A isolated by the vascular fraction (VF) isolation, magnetic-activated cell sorting (MACS) isolation method, and fluorescence-activated cell sorting (FACS) isolation methods were compared with Lonza HUVEC and HMVEC by phase-contrast microscopy; magnification, 40 × .

Techniques: Isolation, FACS, Fluorescence, Microscopy, Produced, Marker, Expressing

Journal: Materials Today Bio

Article Title: Advanced liver-on-chip model mimicking hepatic lobule with continuous microvascular network via high-definition laser patterning

doi: 10.1016/j.mtbio.2025.101643

Figure Lengend Snippet: Construction of continuous microvessels in the laser-patterned microchannels. (A) Schematic illustrations of microvessel formation in the laser-patterned microchannels with different diameters (i.e., Ø50 and Ø80 μm) in the cell-containing hydrogel. RFP-HUVECs were seeded to one side channel of the microfluidic chip. (B) Fluorescence images of RFP-HUVECs (red) in the laser-patterned on days 5 and 9. The cross-view images correspond to the dotted lines (i–iii) in the above image. Scale bars: 200 μm. (C) Quantitative analysis of length of continuous microvessel on days 5 and 9. Data represent the mean ± SD (n = 18/group). ∗ p < 0.05 (two-way ANOVA with the post hoc Tukey's honestly significant difference test). (D) Quantitative analysis of width of microvessel on days 5 and 9. Data represent the mean ± SD (n = 18/group). ∗ p < 0.05 (Student's t -test). Blue lines indicate 50 μm and 80 μm in width of microvessel, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (Basel, Switzerland) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were obtained from PeloBiotech (Planegg, Germany).

Techniques: Fluorescence

Journal: Materials Today Bio

Article Title: Advanced liver-on-chip model mimicking hepatic lobule with continuous microvascular network via high-definition laser patterning

doi: 10.1016/j.mtbio.2025.101643

Figure Lengend Snippet: Construction of whole vascular network in the live-on-chip. (A) Experimental timeline for the fabrication of the liver-on-chip. Three layers of “microvessels” and a vertical “central vein” channel were constructed in the hydrogel region. (B) Fluorescence images of RFP-HUVECs (red) in the laser-patterned microchannels in the 1 st , 2 nd , and 3 rd layers on day 5. Scale bars: 500 μm and 200 μm. (C) 3D images of hepatic lobule-like structure, including HepG2 cells (green) and microvascular network (red), in the hydrogel region on days 5 (upper) and 9 (lower). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (Basel, Switzerland) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were obtained from PeloBiotech (Planegg, Germany).

Techniques: Construct, Fluorescence

Journal: Materials Today Bio

Article Title: Advanced liver-on-chip model mimicking hepatic lobule with continuous microvascular network via high-definition laser patterning

doi: 10.1016/j.mtbio.2025.101643

Figure Lengend Snippet: Liver functionality assays of the liver-on-chip. (A) Schematic illustration of three different groups without laser-patterned microchannels (= Channel (−)), with microchannels (= Channel (+)), and with microchannels vascularized by HUVEC (= Channel (+HUVEC)). (B) The level of albumin production (μg/24 h/chip) on days 5 and 9. (C) The level of urea production (mmol/24 h/chip) on days 5 and 9. Data represent the mean ± SD (n = 6/group). ∗ p < 0.05 (two-way ANOVA with the post hoc Tukey's honestly significant difference test).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (Basel, Switzerland) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were obtained from PeloBiotech (Planegg, Germany).

Techniques:

Journal: Tissue Engineering and Regenerative Medicine

Article Title: A Novel Hypothesis and Characterization to Isolate Microvascular Endothelial Cells Simultaneously with Adipose-Derived Stem Cells from the Human Adipose-Derived Stromal Vascular Fraction

doi: 10.1007/s13770-021-00332-5

Figure Lengend Snippet: Comparative analysis of properties according to the HMVEC-A isolation method. A Morphology and tube formation of HMVEC-A isolated by the vascular fraction (VF) isolation, magnetic-activated cell sorting (MACS) isolation method, and fluorescence-activated cell sorting (FACS) isolation methods were compared with Lonza HUVEC and HMVEC by phase-contrast microscopy; magnification, 40 × . B The VF isolation method produced a significantly higher yield than the FACS isolation method and MACS isolation method (*p < 0.05, **p < 0.01, n = 3). The doubling time was in the order of VF isolation method, MACS isolation method and FACS isolation method, and VF showed the fastest doubling time, but there was no statistically significant difference. The error bars indicate the standard errors of the mean of triplicate measurements. C FACS verification of EC-specific marker expression according to each isolation method. Positivity for the endothelial-specific cell surface antigens CD31 (PECAM1) and CD146 (MCAM) is indicated. Cells were analyzed at passage 2 (n = 3)

Article Snippet: When the most important characteristic of HMVEC-A—i.e., their vascularity—was evaluated, we found that the level of HMVEC-A tube formation was similar to that of Lonza HUVEC and HMVEC.

Techniques: Isolation, FACS, Fluorescence, Microscopy, Produced, Marker, Expressing